ABSTRACT
Recovery in central nervous system [CNS] disorders is hindered by the limited ability of the vertebrate CNS to regenerate and replace damaged myelin, and re-establish functional neural connections. In spinal cord injury and other traumas, demyelination of intact axons is an important factor contributing to loss of function. Previous studies suggest that substantial recovery of function might be achieved through remyelination of damaged axons. This study examined the effects of Triiodothyronine [T3] on in vitro trans-differentiation of adult rat bone marrow stromal stem cells [BMSCs] into oligodendrocyte - like cells [OLCs]. This research was performd experimentally. Under strile conditions, BMSCs were isolated from female sprague-dawley rats. BMSCs were evaluated for different markers such as fibronectin, CD44, CD90, oct4 and CD45. OLCs transdifferentiated from BMSCs by sequential exposure of BMSCs to DMSO and RA in preinduction stage and then induced by heregulin, PDGF-AA, bFGF, and T3 at the induction stage. In both stages of preinduction and induction, the experssion of CD45, CD90, CD44, fibronectin, nestin, Oligo2, O4, NF68, NF160, GFAP, and O1 were assassed by RT-PCR and immunocytochemistry. Our results from immunocytochemistry showed that the fibronectin, CD44, CD90, and CD45 were expressed 94.32 +/- 0.45%, 95.48 +/- 0.24% and 97.16 +/- 0.82% [p< 0.05]. Assessment of of O1, O4 and oligo2 experssion showed that in the induction stage, combination of HRG, PDGF, bFGF and T3 [25ng/ml] have an effective role in transdifferentiation of BMSCs into OLCs. DMSO and RA can transdifferente BMSCs into NeuroProgenitor Cells [NPC] and these cells can be differentiated into OLCs by combination of HRG, PDGF, bFGF and T3 [25ng/m]l.